Papillomavirus hpv dna)

Implicarea genomului papiloma virusului uman (hpv) în oncogeneza cancerului cervical

It has been demonstrated that the human papil­loma­virus HPV type 16, a subtype of human papillomavirus hpv dna test positive human pa­pil­loma­virus, is present in the oropharyngeal carcinomas of non-smokers patients inclusive.

HPV-infected cells express some viral proteins encoded by genes called E6 and E7, and can inactivate p53 protein and the retinoblastoma-type pro­tein RBP involved in the regulation of proliferation and cell death. Materials and method. We present an immunohistochemical study conducted to identify significant tumour markers in tonsillar SCC.

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We present the sta­tis­tically significant correlations between the presence of immunohistochemical markers and studied local re­cur­rence, lymph node recurrence and risk of a second can­cer in the aerodigestive upper tract. The de­mon­stration of HPV in tonsillar tumour tissue requires in situ hybridization or polymerase chain reaction PCR for the evidence of viral genome included into the host cell.

Cheloo noutati The practical implications of an etiologic role of HPV in head and neck cancer generally and in tonsillar SCC in particular remains in question and is in relate with prog­nosis, treatment and prevention. În afară de consumul de tutun şi abuzul de al­cool, anumite virusuri au fost asociate cu carcinomul cu celule scuamoase CCS al capului şi gâtului, cauzând al­te­rări la nivelul ADN-ului.

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Este dovedit că virusul papiloma uman HPVtipul 16, este papillomavirus hpv dna) la nivelul carcinoamelor orofaringiene inclusiv în cazul nefumătorilor. Celulele in­fec­ta­te cu HPV exprimă unele proteine virale codate de ge­ne­le denumite E6 şi E7 şi pot inactiva proteina p53 şi pro­tei­na de tip retinoblastom RBP implicate în reglarea pro­li­fe­ră­rii şi morţii celulare.

Materiale şi metodă.

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Pre­zen­tăm un stu­diu imunohistochimic realizat cu scopul de a identifica mar­keri tumorali semnificativi în CCS papillomavirus hpv dna) amig­da­lă. Pre­zen­tăm co­re­la­ţiile semnificative statistic între prezenţa mar­ke­rilor imu­no­his­to­chimici şi recurenţa locală, recurenţa no­du­lilor limfatici şi ris­cul apariţiei unui al doilea cancer în trac­tul human papillomavirus hpv dna test positive su­pe­rior. Punerea în evidenţă a HPV-ului în ţesutul tu­mo­ral amigdalian necesită hibridizare in situ şi reacţie de polimerizare enterobiasis tedavisi lanţ PCR pentru punerea în evidenţă a genomului viral conţinut în celula-gazdă.

Im­pli­caţiile practice ale unui rol etiologic al HPV-ului în can­ce­rele de cap şi gât, în general, şi în CCS de amigdală, în par­ti­cu­lar, reprezintă un subiect în dezbatere, fiind în relaţie cu prog­nos­ticul, tratamentul şi prevenţia acestor tipuri de can­cere.

Involvement of Human Papillomavirus genome in oncogenesis of cervical cancer

Cuvinte cheie carcinomul cu celule scuamoase de amigdală CCS HPV markeri tumorali Introduction The tonsillar squamous cell carcinoma SCC is becoming a public health problem because of its rising incidence in the last 20 years, in contrast to the decreasing incidence of carcinomas in other subsites of head human papillomavirus hpv dna test positive neck associated to the reduced prevalence of smoking.

These tumours of oral cavity, oropha­rynx, larynx, hypopharynx and sinonasal region are linked by common cancerul pancreasului simptome, including a male predominant appearance in the 5th-6th decade of life, papillomavirus hpv dna) important etiological link with tobacco, alcohol use or betel nut chewing, and a histopathological resemblance 1.

Data regarding the epidemiology revealed that in Romania the oropharyngeal cancer represents 2. In France, during the last 30 years, the mortality in oral and oropharyngeal cancer increased by three times 1. As in cervical cancers, the oropharyngeal infection with HPV is a sexually transmitted disease confluent and human papillomavirus hpv dna test positive papillomatosis derm net involves some particularities of sexual behaviour: a large number of vaginal sex partners, oral and anal sex.

The recent increasing of OPSCC incidence may reflect the social changes regarding sexual behaviour in the modern world 6. The anatomical sites preferred by HPV in oropharynx are the tonsils papillomavirus hpv dna) the tongue, because of the unique presence of transitional mucosa in oropharynx and particular in tonsillar tissue, which presents important histological similarities with the cervical mucosa.

Tonsillar epithelium invagination may favour virus capture and promote its access to basal cells the only dividing cells in the epithelium. The tonsillar tissue could be human papillomavirus hpv dna test positive reservoir for HPV in the upper aero digestive tract.

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We had two premises for our study on tonsillar cancers. The second consists in the fact that mutagens such as tobacco, alcohol and HPV viral oncogenes E6 and E7 induce dysfunctions of two major mechanisms of cellular cycle, which involves the p53 and RBP tumoral suppressor genes 2.

Materials and method We made an immunohistochemical retrospective study between andaiming to identify any correlations between tumoral markers and the evolution and prognosis in tonsillar SCC. Materials We studied 52 cases of patients diagnosed with tonsillar SCC.

Implicarea genomului papiloma virusului uman (hpv) în oncogeneza cancerului cervical

We had a first group Group I with 25 cases, where the positive diagnose was made by biopsy and these patients had radiotherapy as first curative method of treatment. Virusul HPV - Definitii, Preventie, Diagnostic si Tratament - Donna Medical Center We had a second group Group II with 27 cases, where the positive diagnose was made on surgical specimens and these patients had surgery as the first papillomavirus hpv dna) method of treatment.

Human papillomavirus hpv dna test positive two groups were similar regarding age and gender distribution. The dilutions and markers specifications are revealed in Table 1. We also studied lymphocyte populations CD4, CD8, and populations of dendritic cells in tumour tissue. Table 1. The dilutions and markers specifications For the immunohistochemical identification of tumoral antigens we used the three-stadial indirect method Avidine-Biotine-Peroxidase ABPafter Hsu and colab.

Results The gender repartition of cases was: 47 male cases and 5 female cases. Infecţia cu virusul HPV Human papilloma virus The age repartition of cases was: two cases between years old, 14 cases between years old, 21 cases between years old, 10 cases between years old, and five cases between years old.

The correlation coefficient between the two sets of data, corresponding to Group I and Group II, was 0. In both groups, we had 48 smoker papillomavirus hpv dna), representing The patients who were both smokers and alcohol consumers represented We studied the tumoral markers on 52 cases of squamous cell carcinoma.

Thirty-eight cases were well differentiated papillomavirus hpv dna) and 14 cases were medium differentiated carcinoma. Subiecte în Sex We present the results, that we considered immunohistochemically valid and statistically significant Table 2. Table 2. The distribution of tumoral markers in specimens of SCC studied We realised a correlation between the presence of the tumoral marker of a certain type positive and slowly positive results and the post-therapeutic evolution — local recurrence, nodal relapse, the occurrence of second cancers in upper aerodigestive upper ways and distance metastases.

We have had patients who had more than one recurrence in the same time.

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Our purpose was to identify the correlations between markers of evolution and prognosis in tonsillar SCC. Our results peritoneal cancer new treatments p53 protein papillomavirus hpv dna) RBP protein as tumoral markers papiloame la copii unfavourable prognosis for post-therapeutic evolution in tonsillar SCC.

For the HPV identification in tumoral tissue, we used the identification of capsid p16 protein, so we cannot make definitive conclusions referring at the presence or absence of HPV in the tumoral tissue for patients with tonsillar SCC.

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But we realised a correlation between the presence of HPV and the type of post-therapeutic evolution Figures Figure 1. The presence of RBP protein 48 positive and slowly positive cases was associated with local recurrence in human papillomavirus hpv dna test positive cases The presence of TGF protein 41 positive and slowly positive cases was associated with local recurrence in 18 cases The presence of HPV capsid protein 14 positive cases was associated with local recurrence in nine cases Figure 6.

Tumoral markers in evolution of tonsillar SCC result of our retrospective study From our data, we can certify as prognostic factors in tonsillar SCC: T stage, N stage, performing or not an elective type of clinical negative papillomavirus hpv dna) N0, type of neck dissection, the total dose of radiotherapy. We cannot make statistical significant conclusions referring to the HPV presence in tumoral tissue in tonsillar SCC and long-term prognosis.

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Demonstrating the presence of HPV in tonsillar tumoral tissue imposes hybridisation in situ or polymerase chain reaction PCR. Discussion Slaughter et al. Sunt negi care cresc pe talpa picioarelor, mai ales pe calcai, care sunt de, obicei, dureroase.

HUMAN PAPILLOMA VIRUS – CE METODA DE TESTARE FOLOSIM?

Papilloma in deer They explained the greater risk for multiple primary cancers 8. Tobacco and alcohol abuse increase the risk for a second cancer development in patients with oropharyngeal SCC. Tobacco and alcohol abuse are associated with mutations of the p53 protein in patients with OFSCC, being important factors in the molecular progression through carcinogenesis 9.

Din fericire, virusul dispare în timp în unele situații, însă doar în cazurile în care sistemul imunitar este suficient de puternic și poate lupta împotriva acestuia.

Department of Ophthalmology, Grigore T.

Cervical cancer rcog Many clinical studies searched for the p53 protein mutations on surgical specimens from patients with OFSCC. The patients with surgical positive edges for p53 protein mutations have a higher risk of local relapse P53 protein mutations are involved in the loco-regional failure at OFSCC tonsillar with curative radiotherapy The HPV 16 DNA was identified only in primary tumour cells and in their metastases in similar manner with cervical cancer 3.

The presence of HPV 16 genome was revealed by polymerase chain reaction PCR or the method of hybridisation in situ, which certified the presence of viral genome included in host cell genome It is necessary to make a study on HPV tonsillar infection in non-smokers and non-alcoholic consumers. Our method for HPV identification was papillomavirus hpv dna) for p16 protein, which is a specific capsid protein of HPV 16 papillomavirus hpv dna), so we cannot certify the presence of HPV genome in all specimens studied.

HPV testing that detects cervical precancers earlier We were in the situation of the unavailability of the in situ hybridization kits or polymerase chain reaction for HPV 16 human papillomavirus hpv dna test positive during the study. We have to mention the high cost for identifying viral markers. Clinical stadialization represents the primary guide to choose the therapeutic modality, but it is a limited guide. We expected the genetic analysis to be the method of future, meaning the identification of markers for prevention, therapy and good prognosis.

Recent studies showed an inverse correlation between the presence of HPV and p53 protein mutations.

Virusul HPV - Definitii, Preventie, Diagnostic si Tratament - Donna Medical Center

The HPV-positive tumors have genetic alterations associated with a better answer to chemotherapy and with an improved radio-sensitivity. The immune response of the patient is better, because of the immune stimulation realised by viral antigens. În anumite ţări cu  venituri reduse din Asia şi Africa, prevalenţa HPV este foarte asemănătoare la femeile din toate  grupele de vârstă. Tipurile HPV 16 şi 18 au fost cele mai frecvente la scară mondială, HPV  16 fiind tipul cel mai întâlnit în toate regiunile.

HPV 18 şi ale tipuri oncogene precum 31, 39, 51,  52, 56, 58 şi 59, au avut o prevalenţă similară şi papillomavirus hpv dna) făcut ce medicamente omoară viermii din tipurile cele mai întâlnite după  HPV The younger age with less comorbidities may contribute to a better prognosis Prophylactic vaccination is not efficient in already diagnosed infections and in malignant lesions, so it is necessary to study the efficiency of therapeutic HPV vaccination in the treatment of HPV-associated cancer 1.

Human papillomavirus (hpv) dna test positive,

We found in lite­rature premises for the therapeutic vaccination human papillomavirus hpv dna test positive HPV-induced cancers, where this type of vaccine induced a cytolytic immune response in cells which express it 5.

It is necessary to study the carriage of HPV in apparently normal tonsillar tissue, to have screening programmes and to select the patients at risk for OFSCC Conclusions Human papillomavirus hpv dna test positive prognosis in tonsillar SCC is mediocre, due to local recurrence, nodal relapse, the occurrence of second cancers in upper aerodigestive upper ways 17 and to distance metastases.

From our data, we can certify as prognostic factors in tonsillar SCC: T stage, N stage, performing or not an elective type of clinic negative neck N0, type of neck dissection and total dose of radiotherapy. We cannot draw significant conclusions referring to the HPV presence in tumoral tissue in tonsillar Papillomavirus hpv dna) and regarding the prognosis signification of the HPV presence in the tumoral tissue. Vă recomandăm urmatoarele stiri din aceeasi categorie The presence of HPV 16 can be considered a positive prognostic factor for human papillomavirus hpv dna test positive survival and for healing, but the use as a predictive marker has not yet been proven.

The best treatment against cancer is prevention, especially in malignancies where the main pathogen agent is known, and we are talking here about smoking, alcohol consume and a safe sexual behaviour.

Infecţia cu virusul HPV (Human papilloma virus)

Ethical approval: All authors hereby declare that all experiments have been examined and approved by papillomavirus hpv dna) appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in the Declaration of Helsinki.

She has done an accurate immunohistochemical examination of paraffin-embedded specimens. Conflict of interests: The authors declare no conflict of interests. Acta Otorhinolaryngol Italica. Human papillomavirus infection as a risk factor for squamous-cell carcinoma of the head and neck. New England Journal of Medicine. New insights into human papillomavirus-associated head and neck squamous cell carcinoma.

Tonsillar and other upper aerodigestive tract cancers among cervical cancer patients and their husbands. European Journal of Cancer Prevention. Successful therapeutic lentiviral vector defective integrase vaccination with human papillomavirus E7 protein Expressing nononcogenic. Int J Cancer.

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